Coding

Part:BBa_K3726065

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-07)


CDS_AcrbV2

This part corresponds with the protein coding sequence for an Multidrug efflux pump subunit.

The codified gen from Escherichia coli (strain K12), catalyzes for a membrane transporter subunit , which is modified with 6 point mutations that make it specific for butanol and other alcohols.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 331
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 60
    Illegal PstI site found at 331
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 331
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 331
    Illegal NgoMIV site found at 1467
    Illegal NgoMIV site found at 2983
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None